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斑马鱼胚胎的倍性操作与热休克治疗2
JoVE 신문
발생학
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JoVE 신문 발생학
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
DOI:

11:19 min

December 16, 2016

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Chapters

  • 00:05Title
  • 00:47Selecting Females for Egg Collection via Interrupted Mating
  • 01:31Preparation of Sperm Solution
  • 02:50UV Treatment of Sperm Solution
  • 03:37Manual Extrusion of Mature Eggs
  • 04:56In Vitro Fertilization
  • 05:51Heat Shock Treatment
  • 07:13Selection of Embryos with a One-cycle Cytokinesis Stall
  • 08:48Results: Heat Shock 2 Treatment of Zebrafish Embryos
  • 09:57Conclusion

Summary

자동 번역

对于套数操纵修改协议使用热休克诱导胞质一个周期的摊位在早期胚胎。这个协议是表明在斑马鱼,也可以是适用于其它物种。

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