Journal
/
/
La manipulación de ploidía de embriones de pez cebra con el Heat Shock Treatment 2
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
DOI:

11:19 min

December 16, 2016

, , ,

Chapters

  • 00:05Title
  • 00:47Selecting Females for Egg Collection via Interrupted Mating
  • 01:31Preparation of Sperm Solution
  • 02:50UV Treatment of Sperm Solution
  • 03:37Manual Extrusion of Mature Eggs
  • 04:56In Vitro Fertilization
  • 05:51Heat Shock Treatment
  • 07:13Selection of Embryos with a One-cycle Cytokinesis Stall
  • 08:48Results: Heat Shock 2 Treatment of Zebrafish Embryos
  • 09:57Conclusion

Summary

자동 번역

Un protocolo modificado para la manipulación de ploidía utiliza un choque de calor para inducir una parada de un ciclo en la citocinesis en el embrión temprano. Este protocolo se demuestra en el pez cebra, pero puede ser aplicable a otras especies.

Related Videos

Read Article