JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Centre for Translational Omics, Genetics and Genomic Medicine Deptartment, Great Ormond Street Institute of Child Health,University College London, 2Dementia Research Centre, Institute of Neurology,University College London, 3Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy,University of Gothenburg, 4Neurology Unit, Department of Pathophysiology and Transplantation,University of Milan, 5Great Ormond Street Hospital for Children,University College London
Chapters
- 00:05Title
- 01:05Preparation of Standard Peptides
- 03:33LC-MRM Method Development and Data Analysis
- 05:12Results: High-throughput, Multiplex CSF Assay for Quantification of Neurodegeneration Markers and Detection of ApoE Isoforms
- 06:17Conclusion
Se describe un alto rendimiento, múltiplex, y el ensayo de líquido cefalorraquídeo proteómica dirigida (CSF) desarrollado con potencial para la traducción clínica. La prueba se puede cuantificar marcadores potenciales y factores de riesgo para la neurodegeneración, tales como las variantes de la apolipoproteína E (E2, E3 y E4), y medir su expresión alélica.
