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면역학 및 감염병학

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抗体标记用荧光染料使用磁蛋白A和G蛋白珠

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

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抗体标记用荧光染料使用磁蛋白A和G蛋白珠

DOI:

10.3791/54545-v

•

06:48 min

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September 15, 2016

•

Nidhi Nath, Becky Godat, Marjeta Urh

¹Promega Corporation

Chapters

00:05Title
00:41High Capacity Magnetic Protein A or G Bead Antibody Capture
01:43Antibody Labeling Using Amine Chemistry
02:56Antibody Labeling Using Thiol Chemistry
04:46Results: Magnetic BEad Fluorescent Dye Labeling Results in a Highly Efficient Purified Antibody Recovery
05:39Conclusion

Summary

자동 번역

上的胎圈的方法用于标记抗体与小分子使抗体的少量的标记直接从细胞培养基。这种方法是与胺和硫醇化学相容，并且可以并行处理多个样品，手工或使用自动平台。

Tags

Antibody Labeling Fluorescent Dyes Magnetic Protein A Beads Protein G Beads Amine Chemistry Thiol Chemistry Antibody Purification Cell Medium Automated Labeling Manual Labeling Absorbance Measurement Conjugation Buffer Elution Buffer Neutralization Buffer

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