Journal
/
/
El uso de Inducida somática Análisis del Sector (ISSA) para el estudio de genes y promotores implicados en la formación de la madera y el desarrollo del tallo Secundaria
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
DOI:

09:54 min

October 05, 2016

, , , , , ,

Chapters

  • 00:05Title
  • 00:46Preparation of Agrobacterium tumefaciens for Inoculation
  • 01:59Inoculation of Plant Stems with A. tumefaciens
  • 03:44Harvesting for GUS Histological Assay
  • 05:57Identification of Transgenic Tissue
  • 07:22Results: Successful Transformation of Stem Tissue Using the ISSA Method
  • 09:12Conclusion

Summary

자동 번역

Here we present a protocol that facilitates the medium to high throughput functional characterization of gene and promoter constructs in tree secondary stem tissue within comparatively short time frames. It is efficient, easy to use and widely applicable to a range of tree species.

Related Videos

Read Article