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JoVE Journal Immunology and Infection
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
 

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

Article DOI: 10.3791/54581-v • 10:34 min • February 22nd, 2017
February 22nd, 2017 •
1,2, 1,2, 1,2, 1,3,4, 1,2,5
1Center for AIDS Health Disparities Research, Meharry Medical College, 2Department of Biochemistry and Cancer Biology, Meharry Medical College, 3School of Graduate Studies and Research, Meharry Medical College, 4Department of Microbiology and Immunology, Meharry Medical College, 5Tennessee Center for AIDS Research (TN-CFAR), Meharry Medical College

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요약
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This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.

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Keywords: HIV-1 Preintegration Complexes Integration Activity In Vitro Assay SupT1 Cells Retroviral Biology Antiretroviral Therapy Integrase Inhibitors Retroviruses RSV MLV Spinoculation
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