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عزل، التمايز، والكمي للالإنسان الأجسام المضادة إفراز خلايا B من الدم: ELISPOT باعتبارها قراءات الوظيفية للالخلطية الحصانة
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Chapters
- 00:05Title
- 00:48Human Peripheral Blood B Cell Isolation and Purification
- 02:57Naïve and Memory B Cell and Plasmablast/Plasma Cell Sorting
- 03:54In Vitro Isolated Human CD19+, CD19+CD27+ Memory, and CD19+CD27– Naïve B Cell Differentiation and ELISpot Analysis
- 06:18Results: Representative ELISpot Analysis
- 07:06Conclusion
Human peripheral blood is commonly used for the assessment of the humoral immune response. Here, the methods for isolating human B cells from peripheral blood, differentiating human B cells into antibody (Ab)-secreting B cells (ASCs) in culture, and enumerating the total IgM- and IgG-ASCs via an ELISpot assay are described.
