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在拟南芥原生质体转染昼夜的表型有荧光时钟记者的快速分析
JoVE 신문
생물학
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JoVE 신문 생물학
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
DOI:

07:42 min

September 17, 2016

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Chapters

  • 00:05Title
  • 00:39Protoplast Isolation
  • 03:16Protoplast Transfection
  • 05:03Imaging and Data Analysis
  • 05:48Results: Detection of Altered Circadian Rhythms in Arabidopsis Protoplasts
  • 07:02Conclusion

Summary

자동 번역

生物钟的调节有关拟南芥转录组的三分之一,但基因的反馈到报时的比例尚不清楚。在这里,我们可视化的方法来使用一昼夜记者在原生质体瞬时表达的荧光成像拟南芥突变体的任何行快速评估昼夜的表型。

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