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Chapters
- 00:05Title
- 00:52Retinal Organotypic Culture
- 02:31Preparing Samples for Immunostaining
- 04:03Immunostaining
- 05:01Image Analysis
- 06:41Results: The Blood Retinal Barrier is Compromised upon Histamine Exposure and Rescued by LXA4 Treatment
- 08:22Conclusion
A low-cost, easy-to-use and powerful system is established to evaluate potential treatments that could ameliorate blood retinal barrier breach induced by histamine. Blood vessel leakage, Müller cell activation and the continuity of neuronal processes are utilized to assess the damage response and its reversal with a potential drug, lipoxin A4.
