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Messung der Steifigkeit von<em> Ex Vivo</em> Maus Aorten Mit Rasterkraftmikroskopie
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JoVE 신문 생물학
Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy
DOI:

10:35 min

October 19, 2016

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Chapters

  • 00:05Title
  • 00:55Preparing the Mouse and Isolation of the Aorta
  • 02:06Preparing Tissue Samples for the AFM Measurements
  • 02:46Loading the AFM Probe and Aligning the Laser
  • 04:16Calibrating the Deflection Sensitivity and Spring Constant of the AFM Probe
  • 06:28Measuring the Elastic Modulus on Mouse Arteries Ex Vivo
  • 07:50Data Analysis
  • 08:47Results: Determining Aortic Stiffness
  • 09:29Conclusion

Summary

자동 번역

We present detailed protocols for isolation of aortas from mouse and measurement of their elastic modulus using atomic force microscopy.

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