Visualizing the Early Stages of Phagocytosis

Ali Rashidfarrokhi; Veronica Richina; Fikadu G. Tafesse

doi:10.3791/54646

Journal

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생물학

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Visualizing the Early Stages of Phagocytosis

JoVE 신문
생물학

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JoVE 신문 생물학

Visualizing the Early Stages of Phagocytosis

DOI:

10.3791/54646-v

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08:04 min

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February 03, 2017

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Ali Rashidfarrokhi*², Veronica Richina*², Fikadu G. Tafesse²

¹Department of Molecular Microbiology & Immunology,Oregon Health & Science University, ²Ragon Institute of MGH, MIT and Harvard

Chapters

00:05Title
00:38Phagocytosis Binding Assay
03:58Phagocytosis Uptake Assay
04:50Live Imaging of Phagocytosis
06:03Results: Sphingolipids Knockout Cells Show Reduced Phagocytosis
07:03Conclusion

Summary

자동 번역

Here we describe a microscope-based technique to visualize and quantify the early cascades of events during phagocytosis of pathogens such as the fungi Candida albicans and particulates that are larger than 0.5 µm including zymosan and IgG-coated beads.

Visualizing the Early Stages of Phagocytosis

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Chapters

Summary

자동 번역

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