Journal
/
생체공학
/
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

DOI:
10.3791/54648-v

•

07:19 min

•

September 15, 2016

•

, , , , , ,
1School of Physics and Astronomy,University of Exeter, 2Department of Physics and Geology,University of Perugia, 3Istituto Officina dei Materiali del CNR,Unità di Perugia

Chapters

  • 00:05Title
  • 00:46Rat Tail Collagen Fiber Extraction
  • 02:16Bovine Nuchal Ligament Elastin Fiber Extraction
  • 03:44Reflective Substrate Fiber Mounting, Brillouin Experiment Setup and Fiber Spectra Acquisition
  • 04:55Results: Representative Micromechanics of Collagen and Elastin Fibers
  • 06:50Conclusion

Summary

자동 번역

자동 번역

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

Tags

Keywords: Extracellular MatrixCollagenElastinBrillouin SpectroscopyTendonChondroitinase ABCStreptomyces HyaluronidaseTrypsinBovine Nuchal LigamentSodium HydroxideWater Bath

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/54648?article_type=v

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
check_url/kr/54648?article_type=v

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process
check_url/kr/54648?article_type=v

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
check_url/kr/54648?article_type=v

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
check_url/kr/54648?article_type=v

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
check_url/kr/54648?article_type=v

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
check_url/kr/54648?article_type=v

Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
check_url/kr/54648?article_type=v

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
check_url/kr/54648?article_type=v

A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
check_url/kr/54648?article_type=v

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Read Article