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制备及<em>在体内</em>使用基于活动的探针对<em>ñ</em> -acylethanolamine酸酰胺酶

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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

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制备及<em>在体内</em>使用基于活动的探针对<em>ñ</em> -acylethanolamine酸酰胺酶

DOI:

10.3791/54652-v

•

11:01 min

•

November 23, 2016

•

Elisa Romeo, Silvia Pontis, Stefano Ponzano, Fabiola Bonezzi, Marco Migliore, Simona Di Martino, Maria Summa, Daniele Piomelli²

¹Drug Discovery and Development,Istituto Italiano di Tecnologia, ²Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry,University of California, Irvine School of Medicine

Chapters

00:05Title
01:11Preparation of 2-pyridyl Undec-10-ynyl Carbonate and Undec-10-ynyl 2-oxopyridine 1-carboxylate
02:44Preparation of Undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate
04:17Click Chemistry with Azide-PEG3-Biotin
05:14Removal of Excess Click Chemistry Reagents
06:29Streptavidin Enrichment
07:57Click Chemistry with Azide-PEG3-Fluor 545 on Tissue Slices
08:59Results: Synthesis and Applications of NAAA Activity Based Probe, ARN14686
10:21Conclusion

Summary

자동 번역

这里，我们描述一个基于活动的探针的制备和使用（ARN14686，十一碳-10- ynyl- -N - [（3 S）-2-代氮杂环丁烷-3-基]氨基甲酸），允许的检测和定量促炎酶ñ-acylethanolamine酸酰胺酶的活性形式（NAAA）， 在体外和离体。

Tags

Activity-based Probe N-acylethanolamine Acid Amidase (NAAA)Synthesis In Vivo Use Fluorescence Microscopy Inflammatory Diseases Neurodegenerative Diseases Undecynenol DMAP DPC Dichloromethane Sodium Bicarbonate Sodium Sulfate Oxo Asetydenol Ammonium Asetate Diazapropalethylamine

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