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Chapters
- 00:05Title
- 01:11Preparation of 2-pyridyl Undec-10-ynyl Carbonate and Undec-10-ynyl 2-oxopyridine 1-carboxylate
- 02:44Preparation of Undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate
- 04:17Click Chemistry with Azide-PEG3-Biotin
- 05:14Removal of Excess Click Chemistry Reagents
- 06:29Streptavidin Enrichment
- 07:57Click Chemistry with Azide-PEG3-Fluor 545 on Tissue Slices
- 08:59Results: Synthesis and Applications of NAAA Activity Based Probe, ARN14686
- 10:21Conclusion
Ici, nous décrivons la préparation et l' utilisation d'une sonde basée sur les activités (ARN14686, undéc-10-ynyl- N - [(3S) -2-oxoazétidin-3-yl] carbamate) , qui permet la détection et la quantification de la forme active de l'enzyme proinflammatoire N amidase d'acide -acylethanolamine (PNEDA), à la fois in vitro et ex vivo.
