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Teknikker for Imaging prometafase og meta av Meiose jeg i Fast<em> Drosophila</em> oocytter
JoVE 신문
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JoVE 신문 생물학
Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes
DOI:

09:11 min

October 31, 2016

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Chapters

  • 00:05Title
  • 00:36Collection of Late-stage Drosophila Oocytes
  • 02:21Fixation of Oocyte Tissue and Membrane Removal (“Rolling”)
  • 04:09Antibody Staining of Drosophila Oocytes
  • 06:47Results: Visualization of Prophase and Metaphase in Drosophila Oocytes
  • 08:15Conclusion

Summary

자동 번역

We present protocols for the collection, preparation, and imaging of mature Drosophila oocytes. These methods allow the visualization of chromosome behavior and spindle assembly and function during meiosis.

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