/
/
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Physiological Chemistry,University of Veterinary Medicine Hannover, 2Fish Disease Research Unit,University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center,Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ),University of Veterinary Medicine Hannover
Chapters
- 00:05Title
- 01:14Isolation of Human Blood-derived Neutrophils by Density Gradient Centrifugation
- 03:59Isolate the Lipids from the Human Peripheral Blood-derived Neutrophils
- 05:33High Performance Thin Layer Chromatography (HPTLC) to Semi-quantify the Amount of Lipids
- 07:53High Performance Liquid Chromatography (HPLC) to Quantify the Amount of Cholesterol
- 08:48Results: Isolation of Human Blood-derived Neutrophils and Subsequent Lipid Analysis
- 09:33Conclusion
Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation.
