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Chapters
- 00:05Title
- 00:41Preparation and Dissection of Larvae
- 02:34Fixation and Mounting of Larval Fillets
- 04:10Results: Muscle Removal Enables Enhanced Imaging of Da Neurons and the Epidermis
- 06:02Conclusion
Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.
