Journal
/
생화학
/
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

DOI:
10.3791/54672-v

•

08:56 min

•

December 05, 2016

•

, ,
1Department of Cardiology,Chang Gung Memorial Hospital, Chang Gung University College of Medicine

Chapters

  • 00:05Title
  • 01:10RNA Isolation, Qualification, and Quantification
  • 02:29Gel Electrophoresis
  • 03:51Transfer
  • 06:03Detection of N6-methyladenosine
  • 06:50Immunoblotting
  • 07:59Results: Clear Detection of RNA Methylation
  • 08:29Conclusion

Summary

자동 번역

자동 번역

A modified northern blotting method for measuring N6-methyladenosine (m6A) modifications in RNA is described. The current method can detect modifications in diverse RNAs and controls under various experimental designs.

Tags

RNA N6-methyladenosineRNA EpigeneticsNorthern BlottingRNA ModificationsN6A DetectionRNA IsolationAgarose Gel ElectrophoresisMOPS BufferFormaldehydeSSC BufferRNA Transfer

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/54672?article_type=v

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
check_url/kr/54672?article_type=v

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
check_url/kr/54672?article_type=v

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
check_url/kr/54672?article_type=v

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
check_url/kr/54672?article_type=v

A Colorimetric Method for Measuring Iron Content in Plants
check_url/kr/54672?article_type=v

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
check_url/kr/54672?article_type=v

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
check_url/kr/54672?article_type=v

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
check_url/kr/54672?article_type=v

Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
check_url/kr/54672?article_type=v

Quantification of Coenzyme A in Cells and Tissues

Read Article