Journal
/
/
Характеристика Multi-субъединица белковых комплексов человеческого MxA Использование неденатурирующем электрофорез на полиакриламидном геле
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
DOI:

08:55 min

October 28, 2016

,

Chapters

  • 00:05Title
  • 00:48Preparation of Cell Lysate for Non-denaturing PAGE
  • 04:04Electrophoresis
  • 04:53Western Blot
  • 06:34Results: Human MxA Protein from IFN-α Stimulated A549 Cells is a Tetramer
  • 07:22Conclusion

Summary

자동 번역

This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.

Related Videos

Read Article