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Chapters
- 00:05Title
- 01:06Tracheal Exposure
- 02:54Intratracheal Instillation
- 04:27Broncoalveolar Lavage and Lung Processing
- 06:47Results: Representative Post-injury Histopathological and Albumin Leakage Analyses
- 07:40Conclusion
This protocol induces acute lung injury in a mouse that has close fidelity to the pathogenesis of acid pneumonitis observed in humans. We generate a maximal acute nonlethal low pH lung injury and account for differences in rodent-human anatomic respiratory structure using an open tracheostomy coupled with circumferential pressure release.
