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Chapters
- 00:05Title
- 00:50Specifying Parts to be Used in DNA Device Library and Generating Using User/Liquid Handler Instructions
- 02:24Preparing Plasmid DNA and Reagents for Assembly
- 03:55Executing the Assembly Script on the Liquid Handler
- 05:47Transforming the Reactions
- 07:56Clone Verification
- 08:50Results: Automated DNA Assembly Reactions Yield Correct Clones with Comparable Frequency to Reactions Prepared Manually and with Significantly Less Hands-on Time
- 10:20Conclusion
Her, er en automatiseret arbejdsproces kan udføre modulære DNA "enhed" forsamling med en modulær kloning DNA forsamling metode på væske-håndtering robotter præsenteret. Protokollen bruger en intuitiv software-værktøj til at generere flydende handleren valglister for kombinatorisk DNA enhed bibliotek generation, som vi viser ved hjælp af to flydende håndtering platforme.
Tags
Automated DNA AssemblyRobotic Liquid HandlingModular DNA DevicesHigh-throughput DNA ProductionSoftware WorkflowPipetting InstructionsLiquid HandlerDNA Device DesignGenBank FilesDNA PartsCombinatorial DNA AssemblyDNA OverhangsBSA1 EnzymePlate MapsReagent PreparationLiquid Handler Pick ListDNA Assembly Protocol
