This content is Free Access.
Chapters
- 00:05Title
- 00:50Specifying Parts to be Used in DNA Device Library and Generating Using User/Liquid Handler Instructions
- 02:24Preparing Plasmid DNA and Reagents for Assembly
- 03:55Executing the Assembly Script on the Liquid Handler
- 05:47Transforming the Reactions
- 07:56Clone Verification
- 08:50Results: Automated DNA Assembly Reactions Yield Correct Clones with Comparable Frequency to Reactions Prepared Manually and with Significantly Less Hands-on Time
- 10:20Conclusion
Her vises en automatisert arbeidsflyt utføre modulære DNA "enhet" montering med en modulær kloning DNA montering metode på flytende-håndtering roboter. Protokollen bruker en intuitiv programvareverktøy for å generere flytende handler picklists for kombinasjon DNA enheten biblioteket generasjon, som viser vi bruker to flytende håndtering plattformer.
Tags
Automated DNA AssemblyRobotic Liquid HandlingModular DNA DevicesHigh-throughput DNA ProductionSoftware WorkflowPipetting InstructionsLiquid HandlerDNA Device DesignGenBank FilesDNA PartsCombinatorial DNA AssemblyDNA OverhangsBSA1 EnzymePlate MapsReagent PreparationLiquid Handler Pick ListDNA Assembly Protocol
