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Visualizzazione di IL-22 che esprimono Linfociti Utilizzando topi Reporter
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
DOI:

10:30 min

January 25, 2017

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Chapters

  • 00:05Title
  • 01:49Bacterial Artificial Chromosome (BAC) Recombineering of IL-22-tdTomato Vector Generation
  • 03:22Spleen, Thymus, Lymph Node, and Peyer’s Patch Single Cell Suspension Generation
  • 04:48Intestinal Intraepithelial Lymphocyte and Lamina Propria Cell Isolation
  • 06:40IL-22-tdTomato Colitis Expression
  • 07:57Results: Representative Visualization and Localization of IL-22 Receptors
  • 09:31Conclusion

Summary

자동 번역

We describe here a transgenic reporter mouse model to visualize the IL-22-producing cells inside different mouse tissues. This method can be used to track the location of other cytokines or secretary proteins in the mouse.

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