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검출<em> 트리파노소마 (Trypanosoma) brucei</em자기 활성 셀 정렬 및 유동 세포 계측법에 의해> 변형 표면 당 단백질 전환
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:05Sample Harvesting
- 02:05Magnetic Labeling
- 04:05Magnetic Separation
- 05:45Staining and Identification of Contaminants and Switchers
- 07:32Results: Induced Double-strand DNA Breaks Increase the Frequency of Switchers in a Population
- 09:01Conclusion
African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.
