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High-fat Feeding Paradigm for Larval Zebrafish
 

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

Article DOI: 10.3791/54735-v 11:30 min October 27th, 2016
October 27th, 2016

챕터

요약

Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.

Tags

Keywords: Larval Zebrafish High-fat Feeding Fluorescent Lipid Analog Dietary Lipids Lipid Uptake Gastrointestinal Biology Microscopy Lipid Processing Liposomes Cholesterol BSA Egg Yolk Emulsion
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