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Diffusione molecolare nel plasma membrane dei linfociti primari misurati da Fluorescence Correlation Spectroscopy
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:07Starting the FCS System
- 02:26Pinhole Adjustment
- 04:41Measure the Transit Time of the Free Fluorophore
- 05:50Measure the Transit Time of Free Antibody
- 06:51Cell Measurements
- 09:47Results: Assessing the Autocorrelation Curve
- 11:04Conclusion
Tags
Keywords:
Molecular DiffusionPlasma MembranesPrimary LymphocytesFluorescence Correlation SpectroscopyImmunologyCell BiologyNatural Killer CellsMembrane Receptor DynamicsImmunotherapyCancerPrimary CellsCell LinesConfocal Laser Scanning MicroscopeFCS MeasurementsFluorophoresDyePoly-L-lysineCoverglass SlideObjective Lens
