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三维超F-微丝高分辨率显微镜通过干涉光敏定位显微镜（iPALM）

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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

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三维超F-微丝高分辨率显微镜通过干涉光敏定位显微镜（iPALM）

DOI:

10.3791/54774-v

•

11:57 min

•

December 01, 2016

•

Yilin Wang, Pakorn Kanchanawong³

¹Department of Biology,South University of Science and Technology of China, Shenzhen, ²Mechanobiology Institute, Singapore, ³Department of Biomedical Engineering,National University of Singapore

Chapters

00:05Title
00:55Imaging Specimen Preparation
03:11Sample Placement and iPALM Alignment
05:29Calibration of the iPALM Setup
07:47Data Acquisition
09:09Results: Actin Cytoskeleton of Adherent Mammalian Cells Visualized by iPALM with Sub-20 nm Spatial Resolution in 3D
10:55Conclusion

Summary

자동 번역

我们提出了一个协议，用于干涉式光活化的定位显微镜（iPALM），3维单分子定位超分辨率显微镜方法的应用，以便在贴壁哺乳动物细胞的肌动蛋白细胞骨架的成像。这种方法允许的纳米结构特征，否则将通过常规的衍射限制的光学显微镜仍未解决基于光的可视化。

Tags

Keywords: 3D Super Resolution Microscopy IPALM F-actin Filaments Ultrastructural Visualization Single Molecule Localization Cell Sample Preparation Imaging Buffer Cover Glass Assembly Epoxy Sealing Newton’s Rings Pattern

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