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Chapters
- 00:05Title
- 01:10Preparation of the DOTA-type Macrocycle Monomer
- 03:05Preparation of the Dendrimeric Chelator
- 04:55Preparation of the Gd(III) Dendrimeric Constrast Agent (DCA)
- 06:28In Vitro Characterization of a Dendrimeric Contrast Agent (DCA) and Monomeric Analogue (GdDOTA)
- 09:09Results: Dendrimeric Contrast Agent Activity
- 10:38Conclusion
This protocol describes the preparation and characterization of a dendrimeric magnetic resonance imaging (MRI) contrast agent that carries cyclen-based macrocyclic chelates coordinating paramagnetic gadolinium ions. In a series of MRI experiments in vitro, this agent produced an amplified MRI signal when compared to the commercially available monomeric analogue.
