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Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
 

Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins

Article DOI: 10.3791/54786-v 08:24 min November 18th, 2016
November 18th, 2016

챕터

요약

Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.

Tags

Keywords: Primary Endothelial Cells Canine Arteries Canine Veins Cardiovascular Research Angiogenesis Tumor Angiogenesis Atherosclerosis Cell Isolation Cell Culture Gelatin HBSS Collagenase Dispase CECGM Centrifugation
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