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Chapters
- 00:05Title
- 00:53Isolation and Culture of Primary Canine Endothelial Cells
- 04:30Characterization of Canine Aortic Endothelial Cells
- 06:14Results: Canine Primary Endothelial Cell Isolation and Culture
- 07:35Conclusion
Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.
