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Fremstilling af rAAV9 at overudtrykke eller Knockdown gener i mus Hearts
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JoVE 신문 유전학
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
DOI:

11:11 min

December 17, 2016

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Chapters

  • 00:05Title
  • 00:57Transfection of HEK293 with rAAV9 Plasmids
  • 02:14Harvest of Transfected HEK293 Cells and Purification of rAAV9 Vectors
  • 07:04rAAV9 Injections into Neonatal Mice
  • 08:10Results: rAAV9 Overexpression and Gene Knockdown in Mouse Hearts
  • 10:18Conclusion

Summary

자동 번역

In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

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