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Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
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Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

DOI:
10.3791/54805-v

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09:08 min

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February 02, 2017

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, ,
1Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, 2Miller School of Medicine,University of Miami, 3Jerzy Kukuczka Academy of Physical Education

Chapters

  • 00:05Title
  • 00:37Dissection of the Common Carotid Artery and Internal/External Branching
  • 02:53Preparation of the CCA for the MCAO Suture Insertion
  • 03:51Middle Cerebral Artery Occlusion
  • 05:59Reperfusion
  • 07:07Results: Characteristics of Brain Tissue Following Ischemia-Reperfusion
  • 08:38Conclusion

Summary

자동 번역

자동 번역

We describe a mouse model of stroke induced by the occlusion of the middle cerebral artery using a silicone coated suture. The protocol can be applied to induce permanent occlusion or a temporary ischemia, followed by reperfusion.

Tags

Keywords: Ischemic StrokeIschemia-reperfusionMiddle Artery OcclusionInfarct AreaStroke ResearchTreatmentExternal Cerebral ArteryAnesthesiaSurgical ProcedureCommon Carotid ArteryInternal Cerebral ArteryNylon Suture

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