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缺血性中风和缺血再灌注小鼠的诱导使用梗塞区的中心动脉闭塞技术和可视化
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JoVE 신문 의학
Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
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缺血性中风和缺血再灌注小鼠的诱导使用梗塞区的中心动脉闭塞技术和可视化

DOI:
10.3791/54805-v

•

09:08 min

•

February 02, 2017

•

, ,
1Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, 2Miller School of Medicine,University of Miami, 3Jerzy Kukuczka Academy of Physical Education

Chapters

  • 00:05Title
  • 00:37Dissection of the Common Carotid Artery and Internal/External Branching
  • 02:53Preparation of the CCA for the MCAO Suture Insertion
  • 03:51Middle Cerebral Artery Occlusion
  • 05:59Reperfusion
  • 07:07Results: Characteristics of Brain Tissue Following Ischemia-Reperfusion
  • 08:38Conclusion

Summary

자동 번역

자동 번역

We describe a mouse model of stroke induced by the occlusion of the middle cerebral artery using a silicone coated suture. The protocol can be applied to induce permanent occlusion or a temporary ischemia, followed by reperfusion.

Tags

Keywords: Ischemic StrokeIschemia-reperfusionMiddle Artery OcclusionInfarct AreaStroke ResearchTreatmentExternal Cerebral ArteryAnesthesiaSurgical ProcedureCommon Carotid ArteryInternal Cerebral ArteryNylon Suture

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