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Chapters
- 00:05Title
- 00:53Biofilm Formation
- 01:34Resazurin Staining (Plate 1)
- 02:31Crystal Violet Staining (Plate 1)
- 03:49Bactericidal Evaluation
- 04:32Wheat Germ Agglutinin Staining and Matrix Visualization (Plate 2)
- 05:53Staining Viable and Dead Cells for Fluorescent Imaging (Plate 3)
- 06:44Green-to-Red Fluorescent Signal Quantification (Plate 4)
- 07:16Results: Assay Platform Effective for Analysis on Three Endpoints
- 08:56Conclusion
Biofilm infections show high tolerance towards chemotherapy. No single assay captures the complexity of biofilms. Instead, complementary assays are needed. We present a screening platform (developed for S. aureus) that combines assays for viability, biomass, and biofilm matrix. It allows anti-biofilm drug discovery, including the assessment of long-term chemotherapeutic effects.
