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High-throughput Robotisch Assisted Isolatie van temperatuurgevoelige Lethal Mutants in<em> Chlamydomonas reinhardtii</em
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High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
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10:51 min

December 05, 2016

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Chapters

  • 00:05Title
  • 01:17UV Mutagenesis
  • 04:44Identification of ts Mutant Candidates: First Screen
  • 05:34Initial Phenotype Determination
  • 06:41Complementation and Linkage Testing of New Mutants to “Frequent Flyers”
  • 07:47Results: Isolation of Chlamydomonas ts Mutants
  • 09:16Conclusion

Summary

자동 번역

Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.

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