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在脑血管内皮细胞生物能量功能的评价

JoVE 신문
신경과학

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JoVE 신문 신경과학

Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells

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在脑血管内皮细胞生物能量功能的评价

DOI:

10.3791/54847-v

•

06:15 min

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November 19, 2016

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Stephanie L. Rellick^2,4, Heng Hu^3,4, James W. Simpkins⁴, Xuefang Ren^3,4

¹Department of Physiology and Pharmacology,West Virginia University, ²Mitochondrial Evaluation Core,West Virginia University, ³Experimental Stroke Core,West Virginia University, ⁴Center for Basic and Translational Stroke Research,West Virginia University

Chapters

00:05Title
00:36Cerebral Vascular Endothelial (CVE) Cell Culture and Passage
01:48Mitochondrial Functional Assessment
03:27Results: Representative CVE Bioenergetic Function Analyses
05:10Conclusion

Summary

자동 번역

内皮细胞线粒体是维持血脑屏障完整性的关键。我们引入一个协议来测量脑血管内皮细胞生物能量的功能。

Tags

Mitochondrial Function Neurological Disorders Blood-brain Barrier Cerebral Vascular Endothelial Cells Extracellular Flux Cell Culture Cell Medium Trypsin EDTA Cell Seeding Cell Attachment Treatment Mitochondrial Functional Assessment Extracellular Flux Sensor Cartridge Extracellular Flux Assay Medium Bioanalyzer

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