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单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性

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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

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单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性

DOI:

10.3791/54858-v

•

10:23 min

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January 19, 2017

•

Thibaut Perchet, Sylvestre Chea, Milena Hasan, Ana Cumano, Rachel Golub

¹Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur,Institut Pasteur, ²Center for Translational Science,Institut Pasteur, INSERM UMS20

Chapters

00:05Title
00:40Preparing a 96-well Single-cell Sorting Plate
01:55Single-cell Fluorescence-activated Cell Sorting (FACS)
03:02Pre-amplification
03:38Preparing a 96-well Sample Plate
04:23Preparing a 96-well Assay Plate
04:51Preparing and Running the Single-cell Gene-expression Chip
06:52Data Analysis
08:04Results: Single-cell Multiplex RT-qPCR Identifies Cell Heterogeneity of the Liver ILC Compartment
09:33Conclusion

Summary

자동 번역

这个协议描述如何评估在克隆水平大阵列的基因的表达。单细胞RT-qPCR的产生与数百个样品和基因的敏感性较强高度可靠的结果。

Tags

Keywords: Single-cell Gene Expression Multiplex RT-qPCR Heterogeneity Rare Lymphoid Populations Pre-amplification Assay Mix FACS Innate Lymphoid Cells No-input Control CDNA Dilution Reverse Transcription Pre-amplification

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