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暴跌冻结:在透射电子显微镜的超微结构和免疫组化研究工具悬架细胞
JoVE 신문
생물학
Author Produced
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JoVE 신문 생물학
Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
DOI:

13:35 min

May 05, 2017

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Chapters

  • 00:00Title
  • 01:38Preparation of Formvar Grid Film
  • 03:53Preparation of Freeze-substitution Medium
  • 05:06Preparation of Cells
  • 06:11Liquefaction of Propane
  • 07:32Plunge Freezing of Samples
  • 08:32Freeze-substitution
  • 09:04Sample Warming
  • 10:57Results: Plunge Freezing Results in Improved Preservation of the Intracellular Structures
  • 12:50Conclusion

Summary

자동 번역

此手稿描述了通过透射电子显微镜可视化用混悬剂细胞中易于使用和低成本的冷冻固定方法。

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