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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

DOI:
10.3791/54879-v

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08:25 min

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December 25, 2016

•

, , , ,
1Molecular Medicine Branch,NIDDK, NIH

Chapters

  • 00:05Title
  • 00:45Sample Preparation
  • 02:35CL Analyzer Set Up
  • 04:46CL Analyzer Injections and Take-down
  • 06:55Results: Reproducibility of Measurements
  • 07:36Conclusion

Summary

자동 번역

자동 번역

Nitric oxide (NO) is an important signaling molecule in vascular homeostasis. NO production in vivo is too low for direct measurement. Chemiluminescence provides useful insight into NO cycle via measuring its precursors and oxidation products, nitrite and nitrate. Nitrite / nitrate determination in body tissues and fluids is explained.

Tags

Keywords: NitriteNitrateNitric OxideChemiluminescenceBiological SamplesPlasmaHeme ProteinsNitrite-preserving SolutionTriiodideVanadium-chlorideStandard Curve

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