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对于神经化学标记用biocytin填充和加工部分的免疫染色

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Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

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对于神经化学标记用biocytin填充和加工部分的免疫染色

DOI:

10.3791/54880-v

•

08:54 min

•

December 31, 2016

•

Bogumila Swietek, Akshay Gupta, Archana Proddutur, Vijayalakshmi Santhakumar

¹Graduate School of Biomedical Sciences,Rutgers New Jersey Medical School, ²Department of Pharmacology, Physiology and Neuroscience,Rutgers New Jersey Medical School

Chapters

00:05Title
01:19Biocytin-filling during Electrophysiology
03:31Staining of the First Primary Antibody and Biocytin
04:58Staining of the Second Primary Antibody
06:32Results: Successful CCK Staining 1 Week Following the Recovery of Biocytin Staining and CB1R Labeling
07:56Conclusion

Summary

자동 번역

该协议提出了在使用生物胞素填充和随后的免疫组织化学后处理电生理记录修补神经元的形态回收的方法。我们表明，该染色和盖片厚生物胞素填充部分可以与第二初级抗体天或数月后进行复染。

Tags

Biocytin Immunostaining Neurochemical Markers Neuronal Structure Neuronal Function Brain Networks Morphological Recovery Whole-cell Recording Electrophysiology Cell Patching Cell Filling Capacitive Transients Series Resistance Voltage Clamp

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