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Chapters
- 00:05Title
- 00:54Centrifugal Gravity Loading
- 02:22Pellet Culture
- 03:12Micromass Culture
- 04:10Paraffin-embedding Cell Pellets
- 05:59Results: Upregulation of Chondrogenic Differentiation Markers in Adipose-derived Stem Cells Following Centrifugal Gravity Loading
- 07:59Conclusion
Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.
