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Chapters
- 00:05Title
- 00:51Construction of the PUF RNA-binding Domain Scaffold with Customized RNA Binding Specificity by Overlapping PCR
- 02:05Transfection of Engineered Splicing Factors (ESFs) for Modulation of Exon Inclusion and Alternative Splice-site Use
- 03:43Cell Harvest and Identification of Spliced Isoforms
- 06:24Modulation of Endogenous Bcl-x Splicing and Apoptosis by ESFs
- 08:23Results: Subcellular Localization of ESFs in Transfected HeLa Cells
- 09:30Conclusion
Este informe describe un método bioingeniería para diseñar y construir nuevos factores de empalme artificiales (ASFS) que modulan específicamente el empalme de genes diana en células de mamífero. Este método se puede ampliar para diseñar diversos factores artificiales para manipular otros aspectos del metabolismo del RNA.
