JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:51Peripheral Blood Mononuclear Cell (PBMC) Enrichment
- 02:30Monocytes Anti-human CD14 Magnetic Particle Isolation
- 04:08Monocyte Interleukin (IL)-4 and Granulocyte Macrophage–Colony Stimulating Factor (GM-CSF) Stimulation
- 05:10Results: Representative Human Monocyte–Dendritic Cell Generation from Whole Blood
- 06:19Conclusion
Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.
