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使用了大气颗粒物引起的染色体畸变分析<em>体外</em>系统
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Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
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使用了大气颗粒物引起的染色体畸变分析<em>体外</em>系统

DOI:
10.3791/54969-v

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08:48 min

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December 21, 2016

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, , , , ,
1Department of Occupational and Environmental Health,University of Arkansas for Medical Sciences, 2Department of Environmental Health Sciences,University of Alabama at Birmingham, 3Division of Radiation Health,University of Arkansas for Medical Sciences

Chapters

  • 00:05Title
  • 00:35Particle Exposure
  • 02:12Cell Treatment with Particulate Matter
  • 03:21Cell Harvest, Hypotonic Treatment, and Fixation
  • 05:35Slide Cleaning and Metaphase Spread Preparation
  • 07:12Results: Chromosomal Aberrations from Particulate Matter on Metaphase Spreads
  • 08:08Conclusion

Summary

자동 번역

자동 번역

该协议与周围空气的微粒物质处理后描述了在RAW264.7小鼠巨噬细胞体外量化和染色体畸变的表征技术。

Tags

Chromosomal AberrationsParticulate MatterIn VitroAir PollutionCell CultureGenotoxicityMetaphase ArrestTrypsinCell SuspensionCell DensityCell IncubationPBSColcemid

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