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Kultur af murine embryonale Mellemfodsben: en fysiologisk model af endochondral ossifikation
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Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Kultur af murine embryonale Mellemfodsben: en fysiologisk model af endochondral ossifikation

DOI:
10.3791/54978-v

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07:23 min

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December 03, 2016

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, , ,
1Developmental Biology, The Roslin Institute and R(D)SVS,The University of Edinburgh

Chapters

  • 00:05Title
  • 00:51Dissection Preparation
  • 01:47Murine Embryonic Metatarsal Dissection and Culture
  • 04:57Results: A Tool for Understanding Endochondral Ossification
  • 06:20Conclusion

Summary

자동 번역

자동 번역

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

Tags

MurineEmbryonicMetatarsalsEndochondral OssificationEx Vivo ModelPhysiological ModelSkeletal MineralizationDissectionCell-cell InteractionsCell-matrix InteractionsCulture MediumHind LimbMetatarsalTweezersDissecting Microscope

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