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1Section of Nephrology, Department of Medicine,University of Illinois at Chicago, 2Section of Nephrology, Department of Medicine,Jesse Brown Veterans Affairs Medical Center, 3Department of Biology,Kutztown University of Pennsylvania, 4Division of Rheumatology, Department of Medicine,Research Institute of the McGill University Health Centre, 5Department of Microbiology & Immunology,University of Illinois at Chicago
Chapters
- 00:05Title
- 00:49Preparation of Apoptotic BU.MPT Cells
- 03:03Preparation of Necrotic BU.MPT Cells
- 04:39Preparation of BU.MPT Responder Cells
- 05:27Stimulation of Responder Cells with Apoptotic and Necrotic Cells
- 07:03Results: Inhibition of Phosphorylation of GSK3α/β in BU.MPT Responder Cells Following Exposure to Apoptotic Cells Requires Direct Cell-cell Interaction but Is Independent of Phagocytosis
- 08:08Conclusion
Here, we present a protocol for the determination of intracellular signaling events induced in viable cells by physical interaction with adjacent dead or dying cells. The protocol focuses on signaling events induced by receptor-mediated recognition of the dead cells, as opposed to their phagocytic uptake or release of soluble mediators.
