JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:09Introduction
- 01:03Cannulation and Perfusion
- 03:55Purification and Handpicking
- 06:30Troubleshooting: Common Bile Duct Distended During Perfusion
- 07:03Troubleshooting: Duodenum Distended During Perfusion
- 07:37Islet Transplantation at EFP Site
- 10:23Results
- 11:29Conclusion
This protocol demonstrates murine islet isolation and seeding onto a decellularized scaffold. Scaffold-supported islets were transplanted into the epididymal fat pad of streptozotocin (STZ)-induced diabetic mice. Islets survived at the transplantation site and reversed the hyperglycemic condition.
