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支架支持胰岛移植糖尿病小鼠附睾脂肪垫

JoVE 신문
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Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

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支架支持胰岛移植糖尿病小鼠附睾脂肪垫

DOI:

10.3791/54995-v

•

11:57 min

•

July 23, 2017

•

Kai Wang*¹, Xi Wang*¹, Cheng-sheng Han*², Liang-yi Chen, Ying Luo

¹Department of Biomedical Engineering,Peking University, ²Institute of Molecular Medicine,Peking University

Chapters

00:00Title
00:09Introduction
01:03Cannulation and Perfusion
03:55Purification and Handpicking
06:30Troubleshooting: Common Bile Duct Distended During Perfusion
07:03Troubleshooting: Duodenum Distended During Perfusion
07:37Islet Transplantation at EFP Site
10:23Results
11:29Conclusion

Summary

자동 번역

该方案证明了鼠胰岛分离和接种到脱细胞支架上。将脚手架支持的胰岛移植到链脲霉素（STZ）诱导的糖尿病小鼠的附睾脂肪垫中。胰岛在移植部位存活并逆转高血糖状况。

Tags

Islet Transplantation Scaffold-supported Epididymal Fat Pad Diabetes Islet Isolation Collagenase Digestion Histopaque Gradient Beta Cell Replacement Therapy

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