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Chapters
- 00:05Title
- 00:40Injecting Cancer Cells into the Pre-cardiac Sinus of Zebrafish Embryos
- 03:58Scoring Extravasation
- 04:37Mounting Embryos onto Slides and Subsequent Fluorescence Imaging
- 05:34Results: Vascular Invasion of Cancer Cell Lines in Zebrafish Embryos
- 06:50Conclusion
Deze werkwijze gebruikt zebravisembryo's efficiënt testen vasculaire invasieve vermogen van kankercellen. Fluorescerende kankercellen worden geïnjecteerd in de precardiac sinus of dooierzak ontwikkelende embryo's. cel kanker vasculaire invasie en extravasatie wordt beoordeeld via fluorescentie microscopie van de staart regio 24-96 uur later.
