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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro differentiatie van humane CD4 + FOXP3 + geïnduceerde regulatoire T-cellen (iTregs) van naïeve CD4 + T cellen met behulp van een TGF-β-bevattende Protocol
 
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In vitro differentiatie van humane CD4 + FOXP3 + geïnduceerde regulatoire T-cellen (iTregs) van naïeve CD4 + T cellen met behulp van een TGF-β-bevattende Protocol

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

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Dit protocol beschrijft de reproduceerbare productie en fenotypering van mens veroorzaakte regulerende T-cellen (iTregs) van naïeve CD4 + T-cellen in vitro. Verschillende protocollen voor FOXP3 inductie mogelijk maken voor de studie van specifieke iTreg fenotypes verkregen met de respectieve protocollen.

Tags

Immunologie regulatoire T cellen Treg CD4 + T-cellen magnetische celisolatie, cytokines FOXP3 TGF-β IL-2 intracellulaire Flowcytometrie qRT-PCR
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